一测多评法同时测定救乳化毒汤中7种成分的含量OA

The aim of this study was to establish a quantitative analysis of multi-components by single-marker method for the simultaneous determination of Chlorogenic acid,Ferulic acid,Caffeic acid,Chicoric acid,Apigenin,Kaempferol,Formononetin in Jiuru Huadu Tang.The separation was performed on a Shi-madzu Prominence LC-20A column(Agilent Eclipse Plus C18)(100 mm×4.6 mm,2.7 μm)with a gradi-ent elution of water(containing 0.1％phosphoric acid)(A)-acetonitrile(B)as the mobile phase,and the detection wavelength was 220 nm,the column temperature was 40℃,the flow rate was 1.0 mL/min,injec-tion volume of 3 μL.The relative correction factors of the other six components were calculated using chlo-rogenic acid as the internal standard,and their contents were determined.The results showed that the target components exhibited good linearity(r≥0.999 5)over the tested range,with the average spiked recoveries of 97.77％-102.16％and the RSDs of 1.65％-3.26％,The quantitative analysis of multi-components by sin-gle-marker method established in this study for the determination of Chlorogenic acid,Ferulic acid,Caffeic acid,Chicoric acid,Apigenin,Kaempferol,Formononetin in Jiuru Huadu Tang has the advantages of high accuracy and stability,and can be used for the quality control of Jiuru Huadu Tang.