猪流行性腹泻病毒感染IPEC-J2细胞的免疫相关基因筛选OA

The aim of this study is to investigate the changes in the genomic transcription levels of pig epi-demic diarrhea virus(PEDV)LJX strain in vitro,which will provide a reference basis for understanding the role of host immune-related factors in PEDV effection.In this study,we analyzed the transcriptomic changes in IPEC-J2 cells infected with the LJX strain of PEDV and identified immune-related genes and pathways.The optimal infection time of PEDV in IPEC-J2 cells was determined to be 24 hours using Real-Time PCR and indirect immunofluorescence techniques.Subsequently,cell samples infected for 24 hours were selected for transcriptome sequencing to identify differentially expressed genes(DEGs).The results showed that a total of 135 DEGs were identified after PEDV infection,including 77 up-regulated genes and 58 down-regulated genes.Four immune-related genes were identified through GO functional enrichment a-nalysis and KEGG pathway analysis.KEGG analysis found that TLR2 and FOS were significantly enriched in the Toll-like receptor signaling pathway,while CLEC4D and IL23A were significantly enriched in the C-type lectin receptor signaling pathway.The expression trends of DEGs were further validated by Real-Time PCR.This study identified four innate immunity-related genes(TLR2,FOS,CLEC4D,and IL23A)that play crucial roles in defending PEDV infection,providing insights into the mechanisms of host immune responses to PEDV intection.