基于宏基因组分析灞河真核微生物群落结构及氮代谢功能OA

This study employed metagenomic sequencing technology and multivariate statistical methods to systematically analyze the spatial distributions and environmental driving factors of eukaryotic microbial com-munities,as well as their nitrogen metabolism genes at 15 sites along the Ba River.The results indicated sig-nificant differences in the α diversity of eukaryotic microbes among the upper,middle and lower reaches,with higher diversity in the lower reaches rather than that in the upper reaches.In terms of community composition,Bacillariophyta(48.3%)was the dominant phylum,and the relative abundance of Ciliophora remarkably increased in the middle and lower reaches.Nitrogen metabolism in eukaryotes was dominated by assimilation in the upper reaches,with a gradual increase in the abundance of transport-related genes in the middle reaches,and denitrification intensified in the lower reaches.Furthermore,TP,NO3⁻-N and NH4⁺-N significantly affected the eukaryotic microbial community structure,while NH4+-N,NO3⁻-N,Chl a,COND and salinity were the key factors driving the distribution of nitrogen metabolism genes.