基于表型性状与SNP分子标记的山西连翘种质资源遗传多样性分析OA

This study aimed to clarify the degree of fruit phenotypic variation and the characteristics of genetic diversity,population structure,and genetic differentiation of Forsythia suspensa resources in Shanxi,providing an important basis for germplasm conservation and breeding of superior varieties.A total of 46 F.suspensa fruits were collected,and 12 agronomic traits were measured and analyzed.The population genetic structure and genetic diversity of F.suspensa germplasm were evaluated using simplified genome sequencing technology.For the five quality traits of the 46 fruits,the Shannon-Wiener index ranged from 0.631 to 1.074,and the Simpson index ranged from 0.379 to 0.560.The seven quantitative traits exhibited abundant genetic variation,with coefficients of variation ranging from 9.764%(fruit shape index)to 45.494%(forsythin content).Principal component analysis reduced the 12 phenotypic traits to four factors,with a cumulative variance contribution of 74.547%.Sequencing data showed mean Q20 and Q30 values of 98.13%and 94.33%,respectively,with an average GC content of 35.95%.After filtering,a total of 12 347 327 high-quality single nucleotide polymorphism(SNP)loci were obtained.Based on these high-quality SNPs,principal component analysis,population structure analysis,and phylogenetic tree construction were carried out.The 46 germplasm resources were divided into four groups;however,grouping showed little relationship with geographic origin,and intermixing occurred among regions.Mantel test revealed a significant but weak positive correlation between phenotypic and genetic distances(r=0.159,P=0.001).At the molecular level,the four groups exhibited moderate genetic diversity overall,and the genetic differentiation index among populations ranged from 0.027 to 0.084,indicating low to moderate differentiation.The rich genetic diversity of the main phenotypic traits provides a solid material basis for screening superior germplasm and genetic breeding of F.suspensa.