山豆根中异戊烯基黄酮类成分及其细胞毒活性研究OA

Comprehensive chromatographic separation methods were employed to isolate and purify the chemical constituents from the non-alkaloidal fraction of Sophorae Tonkinensis Radix et Rhizoma,and the structures of the compounds were identified with mass spectrometry(MS)and nuclear magnetic resonance(NMR)data.Fourteen prenylated flavonoids were isolated and identified,namely 5-isopentenylsophotonin Ⅰ(1),(2S)-6-isopentenyl-2'-hydroxyglabrol(2),sophoradin(3),sophoradochromene(4),1-prenylpterocarpin(5),sophoranone(6),glabrol(7),7,4'-dihydroxy-6,8-diprenyldihydroflavone(8),isobavachin(9),sophoranochromene(10),5-dehydroxylupinifolin(11),2-[{2'-(1-hydroxy-1-methylethyl)-7'-(3-methyl-2-butenyl)-2',3'-dihydrobenzofuran}-5'-yl]-7-hydroxy-8-(3-methyl-2-butenyl)chroman-4-one(12),ebenosin(13),and wighteone(14).Among them,compounds 1 and 2 were identified as new compounds,the 13 C-NMR data of compounds 3 and 4 were reported for the first time,and compounds 5 and 9 were isolated from this plant for the first time.The MTT assay was used to evaluate the cytotoxic activities of the isolates,and compounds 4,7,and 10 exhibited significant potent cytotoxic activities on HCT116 cells,with IC50 values ranging from 11.44 to 13.43 μmol·L-1,comparable with the positive control 5-fluorouracil(5-FU,IC50 value is 11.70 μmol·L-1).