基于酶解-丙三醇发酵预处理的纤维素厌氧发酵工艺探究OA

Direct inter-species electron transfer(DIET)for methane production eliminates the need for hydrolysis and acid-ification,and electron transfer does not depend on H2 diffusion.This approach overcomes the rate constraints of cellulose hy-drolysis in anaerobic fermentation and the thermodynamic barriers to organic decomposition for acetate production.The addi-tion of glycerol can establish DIET in anaerobic fermentation systems,enhancing methane production efficiency.However,the addition of glycerol requires large doses and incurs high costs.Therefore,this study proposes an anaerobic cellulose fer-mentation process based on enzymatic hydrolysis-glycerol fermentation pretreatment.Results indicate:1)Optimal enzyme loading and cycle for cellulose hydrolysis are 10％and 2 d;optimal temperature,inoculum ratio,and cycle for glycerol fer-mentation pretreatment are 32 ℃,15％,and 9 days.2)The methane production rate in the enzyme-glycerol fermentation pretreatment group reached 1.9 m3·m-3d-1),a 90％increase compared to the untreated group;the organic matter conver-sion efficiency to methane reached 95 L·kg-1VSd-1,a 74％improvement over the untreated group.3)The microbial community in the enzymatic hydrolysis-glycerol fermentation pretreatment group exhibited higher conductivity,greater capacitance,and lower internal resistance.Sphaerochaeta globosa and Methanobacterium formicicum were significantly enriched.