miR-27a-3p调控YAP1/TAZ通路对急性心肌梗死大鼠心功能的影响OA

Objective:To investigate impact of miR-27a-3p on cardiac function in rats with acute myocardial infarction(AMI)by regulating Yes-associated protein 1(YAP1)/transcriptional co-activator with PDZ-binding motif(TAZ)pathway.Methods:Dual luciferase reporter gene assay was used to detect interaction between miR-27a-3p and YAP1.AMI rats were constructed,and success-fully modeled rats were randomly grouped into AMI group,NC agomir group(tail vein injection of NC agomir),miR-27a-3p agomir group(tail vein injection of miR-27a-3p agomir),PY-60 group(tail vein injection of miR-27a-3p agomir+7 μmol/L YAP1 activator PY-60),with 10 rats in each group.Another 10 rats were designated as Sham group,and equal amounts of physiological saline were injected into both Sham group and AMI group.Echocardiography was used to detect cardiac function in rats.ELISA kits were used to detect myocardial damage and inflammatory indicators in rat serum.qRT-PCR was used to detect miR-27a-3p,YAP1 and TAZ mRNA expressions in myocardial tissue.HE staining was used to measure pathological changes of myocardial tissue.TUNEL staining was used to measure myocardial cell apoptosis.Western blot was used to detect YAP1 and TAZ proteins expressions in myocardial tissue.Results:Sham group had intact myocardial tissue structure.AMI and NC agomir groups exhibited marked myocardial disruption,in-cluding myofiber rupture,cellular hypertrophy,interstitial edema,nuclear condensation,and intense inflammation.miR-27a-3p agomir group showed reduced myocardial cell damage and decreased infiltration of inflammatory cells.Myocardial cell damage wors-ened and infiltration of inflammatory cells deepened in PY-60 group.miR-27a-3p could target and negatively regulate YAP1.AMI group had higher LVESD,LVEDD,YAP1,TAZ mRNA and protein expressions,LDH,AST,CK-MB,α-HBDH,IL-1β,TNF-α levels,and apoptosis rate than Sham group,and lower LVEF,LVFS and miR-27a-3p levels than Sham group(P<0.05).miR-27a-3p agomir group had lower LVESD,LVEDD,YAP1,TAZ mRNA and protein expressions,LDH,AST,CK-MB,α-HBDH,IL-1β,TNF-α levels,and apoptosis rate than AMI group and NC agomir group,and higher LVEF,LVFS levels and miR-27a-3p levels than AMI group and NC agomir group(P<0.05).PY-60 group had higher LVESD,LVEDD,YAP1,TAZ mRNA and protein expressions,LDH,AST,CK-MB,α-HBDH,IL-1β,TNF-α levels and apoptosis rate than miR-27a-3p agomir group,and lower LVEF,LVFS and miR-27a-3p levels than miR-27a-3p agomir group(P<0.05).Conclusion:miR-27a-3p may improve cardiac function in AMI rats by regulating YAP1/TAZ pathway.