PLGA-LBP纳米颗粒缓解CIA大鼠炎症及其对肠道菌群的影响OA

Objective:To investigate the therapeutic effects of PLGA nanoparticle-encapsulated Lycium barbarum polysaccha-ride(PLGA-LBP nanoparticles)on joint inflammation in collagen-induced arthritis(CIA)rats and its modulatory role on gut microbiota.Methods:PLGA-LBP nanoparticles were prepared and characterized for their physicochemical properties.A CIA model was estab-lished in SD rats,which were then randomly divided into the following groups:Normal control,model,LBP,methotrexate(MTX),and PLGA-LBP nanoparticle(high-,medium-,and low-dose)groups.From day 8 post-induction,the rats in the respective groups were treated with MTX,LBP,PLGA-LBP nanoparticles.After 35 days,histopathological changes in synovial tissues were assessed by HE staining.Flow cytometric analysis of the percentages of Tfh cells and B cells.Serum levels of IgG,IL-21 and IL-6 were measured using ELISA.Additionally,alterations in the gut microbiota of rats were examined via 16S rRNA sequencing.Results:Compared with the model group,the synovial lesions in the PLGA-LBP nanoparticle group were substantially reduced.The bacteria Trichospirillum,Lactobacillus,and UCG-005 were subject to regulation.PLGA-LBP and LBP both decreased the frequency of Tfh cells and B cells,as well as serum levels of IgG,IL-21 and IL-6;this effect was more noticeable in the PLGA-LBP group.Conclusion:PLGA-LBP nanoparticles can help alleviate CIA by modifying the gut microbiota to decrease joint inflammation.