红芪拆分组分对巨噬细胞吞噬功能的影响OA

Objective:To investigate the effects of fractionation from Hedysari Radix on the immunological function by regulat-ing phagocytic of macrophages.Methods:The fraction of Hedysari Radix was chemically resolved and the content was determined;high performance liquid phase exclusion chromatography-light scattering-differential tandem detection(HPSEC-MALLS-RI)was used to determine the molecular weight of polysaccharides;and studying the antioxidant capacity of split groups in vitro;the effects of frac-tionation from Hedysari Radix on the viability of macrophage were studied by the CCK-8 method.The effects of fractionation on the phagocytosis of macrophage were investigated by flow cytometry.The effects of fractionation on the secretion of nitric oxide(NO),TNF-α,IFN-γ,and IL-1β were detected by Griess assay and ELISA.Results:Hedysari Radix was divided into 7 components:Depro-teopolysaccharides(HPS),segmented polysaccharide HG-2,HG-3,HG-4,ethyl acetate extract(EtOAc),n-butanol extract(nBuOH)and amino acid(AA),the content of flavonoids in the extraction site of EtOAc was 3.80%,the content of saponins in the extraction site of nBuOH was 26.03%,the content of amino acids was 0.40%,and the contents of segmented polysaccharides HG-2,HG-3 and G-4 were 81.35%,88.87%and 82.53%,respectively.The molecular weights of segmented polysaccharides HG-2,HG-3 and HG-4 were 76.49,83.73 and 118.55 kD,respectively.The results of in vitro antioxidant showed that when the sample concentration was 1.0～2.5 μg/mL,the scavenging rate of DPPH free radicals was higher in the EtOAc extract group and nBnOH group(P<0.01).The effects of deproteopolysaccharides and segmented polysaccharides on the scavenging rate and total reducing power of superoxide anion radi-cals were prominent.The 7 fractions had no significant cytotoxicity to macrophages in the concentration range of 12.5～25 μg/mL.Ex-cept for AA,the other six components could significantly enhance the phagocytosis of macrophages to model antigens(P<0.01),among which nBuOH,HPS and EtOAc had astronger effect on macrophage phagocytosis,and the other six components could stimu-late macrophages to secrete NO,TNF-α,IFN-γ and IL-1β(P<0.01).Conclusion:In addition to AA,HPS,segmented polysaccha-ride HG-2,HG-3,HG-4,EtOAc and nBuOH 6 components can activate macrophages,enhance the phagocytic function of macro-phages,thereby enhancing their immune activity,and the activities of nBuOH,HPS and EtOAc are stronger.