大鼠颌骨和股骨骨髓来源间充质干细胞生物学特性的对比研究OA

Objective To explore the differences in biological characteristics between rat orofacial derived mesenchymal stem cells(OMSCs)and bone marrow mesenchymal stem cells(BMMSCs),aiming to provide higher quality seed cells for bone tissue engineering.Methods OMSCs and BMMSCs were extracted from the mandibles and femurs of SD rats,respectively.The surface marker molecules were identified by flow cytometry.Differences in cell proliferation,migration,and osteogenic differentiation between OMSCs and BMMSCs under titanium plate stimulation were compared using the MTT assay,scratch test,RT-PCR,and alizarin red staining.The ability of bone tissue formation in vivo was assessed by heterotopic ossification experiments in nude mice.Results Both OMSCs and BMMSCs met the characteristics of mesenchymal stem cells.However,compared with BMMSCs,OMSCs exhibited a stronger cell proliferation rate and migration ability under titanium plate stimulation.The OMSCs group formed more mineralized nodules,with higher expression of osteogenic-related genes Runx2,ALP,and OCN.The heterotopic ossification experiments further showed that the new bone formation in the OMSCs group was significantly more than that in the BMMSCs group,indicating that OMSCs had a significantly higher osteogenic differentiation capacity than BMMSCs.Conclusion OMSCs exhibit superior biological characteristics which can provide higher-quality seed cells for bone tissue regeneration engineering.