FOXP3、HMGB2在结直肠癌组织中的表达及其与临床病理特征和预后的关系OA

Objective To investigate the expression of forkhead box protein P3(FOXP3)and high mobility group box 2(HMGB2)in colorectal cancer(CRC)tissues,analyze their correlations with clinicopathological features and 1-year postoperative recurrence of patients,and preliminarily screen independent risk factors for early postoperative recurrence in patients with CRC.Methods A total of 70 patients with CRC who received surgical treatment from January 2022 to March 2025 were enrolled as research subjects.Real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the mRNA expression levels of FOXP3 and HMGB2 in cancer tissues and paracancerous tissues of patients.The optimal cut-off value of gene expression was determined and the grouping was completed through the receiver operating characteristic(ROC)curve.The patients were followed up for 1 year after surgery,and the relation-ship between the expression of FOXP3 and HMGB2,and clinicopathological parameters and short-term prog-nosis of the patients was analyzed.Univariate and multivariate Cox regression models were adopted to analyze risk factors for 1-year postoperative recurrence of CRC.Results Paired t-test results showed that the mRNA expression levels of FOXP3 and HMGB2 in CRC tissues were significantly higher than those in paracan-cerous tissues(P<0.05).High expression levels of FOXP3 and HMGB2 were closely correlated with poorly differentiated tumors,invasive depth of T3-T4 stage,lymph node metastasis,distant metastasis and TNM stage Ⅲ-Ⅳ(P<0.05).The proportion of patients with high FOXP3 and HMGB2 expression was markedly higher in those with poor prognosis(recurrence)within 1 year after operation than in patients with favorable prognosis(no recurrence)(P<0.05).Univariate analysis revealed that lymph node metastasis,TNM stageⅢ-Ⅳ,high FOXP3 expression and high HMGB2 expression were all associated with poor postoperative prog-nosis(P<0.05).Multivariate Cox regression analysis verified that lymph node metastasis,TNM stage Ⅲ-Ⅳ,high FOXP3 expression and high HMGB2 expression were independent risk factors for 1-year postoperative recurrence of CRC(P<0.05).Conclusion FOXP3 and HMGB2 are highly expressed in CRC tissues.Their abnormal expression is closely linked to malignant progression and early postoperative recurrence of tumors,and they are expected to serve as potential molecular biomarkers for evaluating the malignant degree and short-term prognosis of CRC.