基于网络药理学与分子技术探讨蒙药巴特日-7味丸治疗溃疡性结肠炎的作用机制OA

Objective To investigate the material basis and mechanism of Mongolian medicine Bateri-Qiwei Pills in the treatment of ulcerative colitis using network pharmacology and molecular docking technology.Methods Medicinal components,drug targets and disease targets of Bateri-Qiwei Pills were screened out by combining published literature and multiple databases including TCMSP,SwissTargetPrediction,ETCM,GeneCards and DISGENET.Venny 2.1.0 was used to find the intersection of the action targets of Bateri-Qiwei Pills in the treatment of ulcerative colitis.Protein-protein interaction(PPI)network of candidate targets was constructed via the STRING platform.GO functional enrichment analysis and KEGG pathway annotation were performed based on the David database.Cytoscape 3.9.1 software was applied to establish the active component-target-signal pathway network,so as to screen the pivotal active components,core targets and key signaling pathways.Results Network pharmacological analysis demonstrated that the core active ingredients of Bateri-Qiwei Pills in the treatment of ulcerative colitis included Morin,Pinocembrin,17-Hydroxy-10,13-Dimethyl-1,2,6,7,8,9,11,12,14,15,16,17-Dodecahydrocyclopenta[A]Phenanthren-3-One,(R)-(6-methoxy-4-quinolyl)-[(2R,4R,5S)-5-vinylquinuclidin-2-yl]methanol and Cheilanthifoline.Core thera-peutic targets contained AKT1,TNF,GAPDH,IL-6 and IL1B.Molecular docking verified the binding activ-ity between pharmacodynamic components and core targets of Bateri-Qiwei Pills.Conclusion Mongolian medicine Bateri-Qiwei Pills exerts therapeutic effects on ulcerative colitis through the synergistic action of multi-components,multi-targets and multi-signaling pathways.