枣TIFY基因家族鉴定及非生物胁迫下的表达分析OA

The TIFY gene family is a class of plant-specific transcription factors that play key roles in abiotic stress responses.To investigate the characteristics of the TIFY gene family in Ziziphus jujuba Mill.and its function in stress resistance,this study identified TIFY family members based on the'Junzao'genome using bioinformatics methods,and analyzed their physicochemical properties,phylo-genetic relationships,gene structures,and promoter cis-acting elements.Furthermore,qRT-PCR was used to examine the expression patterns of these genes in tissue-cultured seedlings of'Junzao'and'Wild jujube'under salt-alkali,drought,and high-temperature stresses.A total of 18 TIFY genes were identified and classified into four subfamilies:TIFY,PPD,ZML,and JAZ.Promoter analysis revealed an abundance of hormone-and stress-related cis-acting elements.Expression profiling demonstrated that ZjJAZ1 and ZjTIFY1 are core regulators in abiotic stress responses.ZjJAZ1,a broad-spectrum stress-responsive gene,was rapidly and strongly induced in wild jujube under all three stresses,where-as ZjTIFY1 played significant roles in salt-alkali and high-temperature stresses.Additionally,wild ju-jube exhibited a stronger overall stress response than'Junzao',with its TIFY genes-especially those in the JAZ subfamily—showing more activated genes,higher expression amplitudes,and longer re-sponse durations.In contrast,'Junzao'responded more slowly and weakly,though its roots displayed relative sensitivity to high-temperature stress.This study systematically reveals the characteristics of the jujube TIFY gene family and its differential expression under abiotic stresses,providing important candidate genes and a theoretical foundation for molecular breeding of stress-resistant jujube varieties.