枣胚胎发育蛋白质分析及氨基酸代谢通路关键蛋白筛选OA

Embryo dysplasia or abortion is a prevalent disorder in jujube(Ziziphus jujuba Mill.)em-bryos is a common phenomenon,which seriously restricts jujube cross breeding.Proteins are closely related to embryo development.Currently,systematic analyses of proteins involved in amino acid me-tabolism during embryo development in jujube varieties with different fertility levels are lacking.To explore the protein composition and amino acid expression differences in jujube embryos at different developmental stages,this study employed data independent acquisition(DIA)technology to perform Gene Ontology(GO)functional annotation of differential proteins,Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathway annotation,and cluster analysis of highly embryo-fertile'Sour jujube'(Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chow)and low embryo fertility cul-tivar'Kongfusucui jujube'at three different developmental stages.The results showed that a total of 10 516 proteins were identified,among which 1 558 proteins exhibited significant differential expres-sion.Among these differential proteins,1 337 had catalytic activity,and 261 proteins were involved in amino acid synthesis and metabolism.GO enrichment analysis revealed that the differentially ex-pressed proteins with catalytic activity were mainly enriched at 30 and 50 days after embryo formation.The expression levels of serine acetyltransferase(XP_015865688.1),serine hydroxymethyltransferase(XP_015868935.1),and 3-phosphoshikimate 1-carboxyvinyltransferase(XP_015888682.1),which are involved in amino acid metabolism,showed a continuous increase during embryo development in em-bryo-fertile'Sour jujube',while in embryos of the low-fertility cultivar'Kongfusucui jujube',their expression first decreased and then increased.In particular,serine acetyltransferase(XP_015865688.1)was significantly down-regulated at the peak of abortion(40 days after embryo formation)during the KD4 vs.SD4 period,suggesting that it plays a key role in regulating amino acid accumulation.