藜麦CqZIP基因家族鉴定与锌铁缺乏时表达模式分析OA

Zinc-iron transporter(ZIP)proteins are widely distributed metal ion transporters in plants and play crucial roles in the uptake and transport of zinc and iron ions.In this study,based on the qui-noa genome database,we used bioinformatics methods to identify and analyze the characteristics of the CqZIP gene family and also explored the expression patterns of CqZIP genes under iron or zinc defi-ciency conditions using real-time quantitative PCR(qRT-PCR).The results revealed that the quinoa CqZIP gene family contains 28 members,named CqZIP1-CqZIP28,which are divided into 4 subfami-lies and are unevenly distributed across 13 chromosomes of quinoa.The encoded proteins range from 175 to 863 amino acids in length,with molecular mass of 18 847.87 to 94 600.79 u and isoelectric points(pI)of 4.84 to 9.25.Among the 28 CqZIPs,24 CqZIP proteins are localized in the plasma mem-brane.Most CqZIPs contain transmembrane domains except for CqZIP26.Transcriptome analysis of different tissues in quinoa showed that CqZIP expression was tissue-specific.CqZIP9 exhibited the highest expression level in roots,while CqZIP22 showed higher expression levels in stems and leaves.Transcriptome data from roots under zinc deficiency conditions showed the expression levels of 9 CqZ-IP genes was upregulated and that of 4 CqZIP genes was downregulated.qRT-PCR results showed that during recovery from zinc deficiency,compared with the control,the expression levels of CqZIP5,CqZIP9,and CqZIP12 was up-regulated in roots,stems and leaves.These results suggest that these three genes may be involved in the uptake,long-distance transport and redistribution of Zn2+.