氨基寡糖素和复配药剂诱导甜瓜抗枯萎病研究OA

[Objective]In this study,the optimal combination of oligosaccharins and mannose-oligosaccharides,fructooligosaccharides,xylose-oligosaccharides,sodium silicate and salicylic acid were selected,and the green protection technique was established to control the damage of Fusarium oxysporum f.sp.melonis on melon industry.[Methods]The optimal combination of oligosaccharins and compound agents were selected by the dual culture technique,control effect of pot and field experiments,the total chlorophyll content and defense enzyme(PAL,POD,CAT)activity detection in melon leaves against Fusarium oxysporum f.sp.melonis and so on.[Results]The results showed that the inhibition rate of mycelium growth and spore germination of Fusarium.oxysporum f.sp.melonis were the highest by oligosaccharins,and better effect in mycelial growth inhibition by salicylic acid,and better effect in spore germination inhibition by mannose-oligosaccharides,xylose-oligosaccharides,and sodium silicate,while no inhibitory effect of mycelial growth and spore germination by fructooligosaccharides.The optimal antibacterial formulation ratios were 9∶1 by oligosaccharins combined with mannose-oligosaccharides,sodium silicate,and salicylic acid,and 7∶1 by oligosaccharins combined with xylose-oligosaccharides.The relative control effect of pot and field experiments were over up to 30％by oligosaccharins and six agents individual treatment,and was the highest by oligosaccharins,which was over up to 50％in both environments,and salicylic acid and sodium silicate were over up to 40％.After combination of oligosaccharins treatment,it showed that the relative control effect of pot experiments were over up to 40％by sequential induction and simultaneous induction treatments.The relative control effect of field experiments were all over up to 40％by the combination of oligosaccharins with salicylic acid,and by combination of oligosaccharins with sodium silicate.The relative control effect of the sequential induction of pot and field experiments were the highest by combination of oligosaccharins with salicylic acid,up to 66.23％in pot and 59.38％in fields,and were significantly better to individual agent and other compounding combinations.The growth promoting effects were the most significant by combination of oligosaccharins with salicylic acid,and individual induction by salicylic acid,as well as sodium silicate,and the plant height and stem diameter of melon seedlings were increased significantly,while that were the highest of chlorophyll content,POD,PAL and CAT enzyme activities of melon leaves,and total fruit weight and average weight of melon field.[Conclusion]The optimal combination of oligosaccharins and salicylic acid could be effectively activate the defence response of melon,and prevent pathogen infection to provide a new technique for the green control of melon Fusarium wilt.