外源褪黑素对盐胁迫下柳兰幼苗生长及生理特性的影响OA
Effects of Exogenous Melatonin on Growth and Physiological Characteristics of Chamerion angustifolium Seedlings under Salt Stress
[目的]土壤盐渍化是制约全球园林绿化植物生长发育的主要非生物胁迫因子之一.褪黑素(Melatonin,MT)是一种广泛存在于生物体内的吲哚胺类调节剂,通过强化植物胞内抗氧化防御体系、清除多余活性氧以及维持离子平衡,在缓解干旱、盐渍及极端温度等逆境伤害中展现出显著功效.探究在盐胁迫下,外源MT对柳兰(Chamerion angustifolium L.Scop)幼苗的生长发育及生理稳态的调控机制.[方法]以锡林郭勒(种源A)和呼伦贝尔(种源B)野生柳兰幼苗为材料,采用盆栽试验,设置盐胁迫处理(0,150 mmol·L-1 NaCl),研究叶面喷施不同浓度MT(0,100,200 μmol·L-1)对幼苗表型及生理特性的影响.[结果]150 mmol·L-1 NaCl胁迫显著抑制柳兰的生长及光合固碳效率.喷施200 μmol·L-1 MT可显著缓解盐害效应:2个种源幼苗的生长抑制均得到缓解.外源MT处理通过提高SOD、POD、CAT、GR、APX和GPX等抗氧化酶活性,增强2个种源柳兰幼苗的酶促抗氧化防御能力,降低MDA和T-ROS积累,从而减轻盐胁迫引起的氧化损伤.此外,MT提高Rubisco和Rubisco活化酶(RCA)活性,修复盐胁迫下柳兰幼苗的碳代谢过程.[结论]外源MT处理可通过缓解盐胁迫下柳兰幼苗的生长抑制、改善光合碳代谢过程并强化抗氧化防御能力,显著提高柳兰幼苗的耐盐性.
[Objective]Soil salinization is one of the major abiotic stress factors limiting the growth and development of landscape plants worldwide.Melatonin(MT),an indoleamine regulator widely presents in living organisms,has shown considerable potential for alleviating damage caused by drought,salinity,and extreme temperatures by enhancing intracellular antioxidant defense,scavenging excess reactive oxygen species,and maintaining ionic homeostasis.This study aimed to investigate the regulatory mechanisms of exogenous MT on the growth,development,and physiological homeostasis of Chamerion angustifolium seedlings under salt stress.[Methods]Wild Chamerion angustifolium seedlings from Xilingol(provenance A)and Hulunbuir(provenance B)were used in a pot experiment.Two NaCl concentrations(0 and 150 mmol·L-1)and three foliar MT concentrations(0,100,and 200 μmol·L-1)were applied to evaluate the effects of MT on seedling phenotypic and physiological traits.[Results]Exposure to 150 mmol·L-1 NaCl significantly inhibited the growth and photosynthetic carbon fixation efficiency of Chamerion angustifolium seedlings.Foliar application of 200 μmol·L-1 MT significantly alleviated salt-induced damage,and growth inhibition was mitigated in seedlings of both provenances.Exogenous MT enhanced enzymatic antioxidant defense in seedlings of both provenances by increasing the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),glutathione reductase(GR),ascorbate peroxidase(APX),and glutathione peroxidase(GPX),while reducing the accumulation of malondialdehyde(MDA)and total reactive oxygen species(T-ROS),thereby mitigating salt-induced oxidative damage.In addition,MT increased the activities of ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)and Rubisco activase(RCA),thereby promoting the recovery of photosynthetic carbon metabolism in Chamerion angustifolium seedlings under salt stress.[Conclusion]Exogenous MT significantly improved the salt tolerance of Chamerion angustifolium seedlings by alleviating salt-induced growth inhibition,improving photosynthetic carbon metabolism,and strengthening antioxidant defense capacity.
宝秋利;刘越
内蒙古农业大学 林学院,呼和浩特 010019||内蒙古农业大学 职业技术学院,内蒙古 包头 014109内蒙古农业大学 林学院,呼和浩特 010019
农业科技
柳兰盐胁迫褪黑素光合系统抗氧化防御
Chamerion angustifoliumsalt stressmelatoninphotosynthetic systemantioxidant defense
《沈阳农业大学学报》 2026 (4)
1-11,11
国家重点研发计划课题项目(2023YFF1304102)国家自然科学基金项目(32160335)
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