兔耳缘静脉留置导管相关性血栓形成的病理时间动态研究OA

Objective:To investigate the dynamic histopathological evolution of catheter-related thrombosis(CRT)in rabbit marginal ear veins within 12-96 hours,and identify the critical time point for the initiation of thrombus organization.Methods:Fifty New Zealand rabbits were divided into five indwelling groups with catheter retention durations of 12 h,24 h,48 h,72 h and 96 h(8 rabbits per group,with an additional 2 puncture-only control rabbits for each group).Semi-quantitative scoring was performed to evaluate thrombus characteristics,inflammatory response and vascular injury after hematoxylin-eosin(HE)staining.Results:The incidence of CRT rose from 25.0%at 12 h to 100.0%at 96 h(P<0.001).Scores for thrombus area,composition and adhesion stability increased significantly over time(all P<0.05).The dominant inflammatory cell shifted from neutrophils to macrophages,and vascular injury progressed from endothelial denudation to elastic lamina rupture.The total thrombus score was positively correlated with the total inflammatory score(r=0.89,P<0.001).Conclusion:The pathological progression of CRT in rabbit marginal ear veins is time-dependent.The 48-hour time point may serve as a critical turning point for early thrombus organization and aggravated vascular injury,providing experimental histopathological evidence for determining the safe indwelling duration of peripheral intravenous catheters in clinical practice.