绞股蓝提取物对巴马香猪肠道健康的影响OA

The experiment aimed to investigate the effects of Gynostemma pentaphyllum extract(GPE)on the intestinal health of weaned Bama miniature pigs.A total of 50 weaned Bama miniature piglets aged 30 to 35 days with an initial body weight of(5.34±0.25)kg were randomly divided into five groups,with five replicates per group and two piglets per replicate.The control group(CON group)was fed a basal diet,the experimental groups were fed the basal diet supplemented with 0.4%,0.8%,and 1.6%GPE,respectively,and the Astragalus extract(APE)group was fed the basal diet supplemented with 0.2%APE.The pre-test period lasted for seven days,and the formal test period lasted for 28 days.The results showed that compared with the CON group,the 0.8%GPE and 1.6%GPE groups significantly improved the morphological structure of various segments of the small intestine.The mRNA expression levels of Toll-like receptor 4(TLR4)and tumor necrosis factor receptor-associated factor 6(TRAF6)in the jejunum and ileum of the experimental groups were significantly down-regulated(P＜0.05).In the 1.6%GPE group,the mRNA expression levels of interleukin-10(IL-10)in the ileum,as well as Toll-like receptor 9(TLR9),tight junction protein 1(ZO-1),and occludin(OCLN)in the jejunum were significantly up-regulated(P＜0.05).Compared with the CON group,the relative abundance of Firmicutes in the cecum was significantly increased(P＜0.05),the relative abundance of Verrucomicrobiota in the cecum and Streptococcus in the colon were significantly decreased of the experimental groups(P＜0.05).The relative abundance of Bacteroidetes in the colon of the 0.4%GPE and 1.6%GPE groups was significantly increased(P＜0.05),and the relative abundance of Streptococcus in the cecum was significantly decreased(P＜0.05).The study shows that GPE can improve intestinal morphology,regulate inflammatory responses,and optimize microbiota structure in Bama miniature pigs,and the recommended supplementation dose is 1.6%.