姜黄素和咖啡酸对鼠伤寒沙门氏菌感染文昌鸡的保护作用OA

The experiment aimed to investigate the effects of curcumin(CUR)and caffeic acid(CA)on the growth performance,antioxidant capacity,intestinal barrier function,and inflammatory response of Wenchang chickens infected with Salmonella typhimurium(ST).A total of 180 male Wenchang chickens aged one day were randomly divided into six groups,namely control group(group CON),ST challenge group(group ST),ST+5 mg/kg ciprofloxacin group(group ST+CIP),ST+200 mg/kg CUR group(group ST+CUR),ST+100 mg/kg CA group(group ST+CA),ST+200 mg/kg CUR+100 mg/kg CA group(group ST+CUR+CA),with three replicates in each group and 10 chickens in each replicate.The test period lasted for 21 days.The results showed that compared with the group ST,the average daily gain(ADG)from 15 to 21 days in groups ST+CUR,ST+CA,and ST+CUR+CA was significantly increased(P<0.05),the feed to gain ratio(F/G)was significantly decreased(P<0.05).On the 4th and 11th day after toxin attack,compared with the group ST,the villus height,villus height to crypt depth ratio,goblet cell number in groups ST+CUR,ST+CA,and ST+CUR+CA were significantly increased(P<0.05).The expression of tight junction protein and mucin 2(MUC2)in the ileum were significantly upregulated(P<0.05).The expression of interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)were significantly downregulated(P<0.05).The activity of superoxide dismutase(SOD)on the 4th day after toxin attack was significantly increased in the groups ST+CUR and ST+CA than in the group ST(P<0.05).The expression of cyclooxygenase-2(COX-2)on the 4th day after toxin attack and the content of malondialdehyde(MDA)on the 11th day after toxin attack were significantly reduced in the groups ST+CUR,ST+CA,and ST+CUR+CA(P<0.05).The study shows that CUR,CA and their combination can effectively alleviate the growth inhibition and intestinal injury of Wenchang chickens caused by ST toxin attack by enhancing antioxidant capacity,reducing intestinal inflammation and improving intestinal barrier function,the combination of the two has a better effect on improving intestinal mucosal barrier function and intestinal inflammatory response in the early stage of infection.