薰衣草LabHLH35基因的克隆及表达分析OA

Lavender(Lavandula angustifolia)possesses significant ornamental value due to its unique floral coloration and aromatic properties.Flower color is a key determinant of its ornamental value,and antho-cyanin is the core matter influencing flower color.The bHLH transcription factors play crucial roles in regula-ting anthocyanin biosynthesis pathways in plants.To elucidate the regulatory mechanisms of anthocyanin bio-synthesis in lavender and identify candidate genes for novel cultivar breeding,the LabHLH35 gene was cloned from lavender floral organs and the bioinformatics analysis,tissue-specific expression profiling,and yeast self-activation assays were conducted in this study.The length of LabHLH35 gene was 678 bp,encoding a 225-a-mino acid protein classified as an unstable hydrophilic protein.The LabHLH35 protein contained a conserved basic Helix-Loop-Helix(bHLH)domain,showing close phylogenetic relationships with SdbHLH35(Salvia divinorum)and SsbHLH35(Salvia splendens)proteins.Subcellular localization confirmed its nuclear localiza-tion,and yeast assays demonstrated its transcriptional self-activation activity.Expression analysis revealed that LabHLH35 was expressed across various tissues and floral developmental stages,with the highest expression level observed in flowers,consistent with more anthocyanin accumulation in flowers,indicating a distinct tis-sue-specific expression pattern.This study provided preliminary insights into the functional characteristics of the LabHLH35 gene and could lay the foundation for further investigation on the regulatory role of the LabHLH35 transcription factor in lavender anthocyanin synthesis.