反胶束液液微萃取在亚甲基蓝检测中的应用OA

Methylene blue is a water-soluble dye.Water polluted by methylene blue can induce cancer,cause environmental pollution,and lead to ecological disaster.Reverse micelle mediated dispersive liquid-liquid microextraction(RM-DLLME)is a simple,rapid and environmentally friendly novel sample preparation technique.In this study,RM-DLLME combined with spectrophotometry is used to detect methylene blue in water.After optimizing extraction conditions,for example changing the types and concentrations of surfactants,enrichment optimization is carried out to form a new method for detecting methylene blue in water by reverse micelle mediated dispersive liquid-liquid microextraction combining with spectrophotometry.Vreverse micelle solvent(3.1 mmol/L sodium dodecyl sulfate-isoamyl alcohol solution):Vmethylene blue aqueous solution(pH 4.8∶)Vmethanol=2.0∶27.5∶2.5,after vortex mixing and centrifugation,the upper phase is taken for detection at 659.0 nm.The limit of detection(LOD)and the limit of quantitation(LOQ)of the new method are 0.7 μg/mL and 2.2 μg/mL,the enrichment fator is 15.0,and the relative standard deviation(RSD)is 4.2％,meanwhile the recoveries are 90.1％～96.8％.The results show that the new method has low LOD and LOQ,good enrichment effect,small matrix effect in 5 kinds of waters,and wide application range.