红九棘鲈单拷贝基因保守序列筛选及其在种质鉴定中的应用OA

The tomato hind,Cephalopholis sonnerati,is a new species of high economic value grouper,which is widely distributed,rich genetic diversity,and difficult to identify its germplasm.In order to develop molecular markers and methods suitable for germplasm identification of the tomato hind,this study used the single copy gene database of spinal animals combined with the resequencing data of the tomato hind to screen the highly conserved sequences of the tomato hind.A target sequence located in the single-copy gene ceramide glucosyltransferase(ugcg)was successfully selected,and specific primers were designed.A specific band of 226 bp with only individual base differences could be amplified from the tomato hind,but the corresponding band cannot be amplified from other groupers.In this study,a single copy conserved gene was selected as the core tool to establish a rapid and accurate identification method for the germplasm resources collection,identification and evaluation and also laid a foundation for the molecular breeding technology innovation and the artificial breeding of the tomato hind.