StCBF/DREB1家族鉴定及StDREB4对温度胁迫下马铃薯生长和抗氧化能力的影响OA

Potato(Solanum tuberosum)is a cool-season crop with low tolerance to low temperatures,and high temperature severely inhibits its growth and development.As plant-specific transcription factors,CBF/DREB1(C-repeat binding factor/dehydroresponsive element binding)play vital roles in improving plant stress resistance.To understand the characteristics and functions of the CBF/DREB1 family members(StDREBs)of potato,this study conducted a systematic identification and analysis of StDREBs on a whole-genome scale,and detected their expression patterns under heat and cold stresses.The results showed that there were 8 StDREBs in potato,with 207～329 amino acid residues,the molecular weights of StDREBs ranged from 23.421 to 36.720 kD,containing 6～8 motifs,none of which contained transmembrane domains,signal peptides and introns.StDREBs contained 20～94 phosphorylation sites,the isoelectric points were all less than 7,the aliphatic coefficients were all less than 80,the instability coefficients were all greater than 40,and the grand average of hydropathicity was all less than 0.By detecting the expression patterns of StDREBs under heat and cold stress,it was found that compared with the control group,the expression level of StDREB4 significantly increased at 6,12,24 and 48 h of the stress treatment,suggesting that it may be related to the heat and cold stress response process of potatoes.In addition,by constructing potato transgenic lines with overexpression of StDREB4 and further analyzing the functions of StDREB4,it was found that overexpression of StDREB4 enhanced the heat and cold tolerance of potato by regulating the activity of antioxidant enzymes and reducing the level of reactive oxygen species,thereby promoting the growth of transgenic potato lines under heat and cold stresses.In conclusion,this study provides candidate gene resources for improving the heat and cold tolerance of potatoes,which are conducive to enhancing the yield and quality of potatoes.