ATP在线检测设备研发、性能评估及应用研究OA

Adenosine triphosphate(ATP)serves as the primary energy currency of cells,and changes in its content reflect the activity level of microorganisms.However,the lack of online detection techniques has greatly restricted the popularization and application of this indicator in relevant fields.To address this issue,this study established an online ATP detection method and developed a corresponding online ATP detection device.The performance of the instrument was evaluated in terms of its detection limit,stability and accuracy.Subsequently,this instrument was applied to two categories of biological reaction processes:a kitchen wastewater bioreactor and a Bacillus fermentation process,with ATP data obtained to analyze its correlations with pollutant removal efficiency and bacterial biomass,respectively.The results showed that the photon detection limit of the device was 174RLU～2×107 RLU;repeated measurements of ATP standard solutions at different concentrations yielded a minimum relative deviation of 3.30%,indicating good stability of the device.The detection values of the device for the same samples exhibited a linear relationship with those obtained using a microplate reader(R2=0.970 4),demon-strating high detection accuracy of the device.The ATP response trends of activated sludge in kitchen wastewater and Bacillus revealed that ATP concentration could effectively character-ize the system treatment efficiency and microbial metabolic activity.Moreover,ATP preceded bacterial biomass in indicating the entry of the microbial community into the active growth phase.