外用雷公藤提取物对皮炎小鼠模型表皮通透屏障的影响OA

Objective To investigate the effects of Tripterygium wilfordii extract(TWE)on the epidermal permeability barrier in mice with skin inflammation.Methods A total of 20 C57BL/6J mice were randomly divided into four groups:normal control group,12-O-tetrade-canoylphorbol-13-acetate(TPA)group,TPA+vehicle group,and TPA+0.3％TWE group.Ex-cept for the normal control group,mice in the other groups were induced to develop irritant contact dermatitis using TPA.The treatment groups were topically administered with vehicle and 0.3％TWE,respectively.Another 20 C57BL/6J mice were randomly assigned to a normal control group,1-fluoro-2,4-dinitrobenzene(DNFB)group,DNFB+vehicle group,and DNFB+0.3％TWE group.Mice in all groups except the normal control group were established with allergic con-tact dermatitis models via DNFB application.The treatment groups received topical vehicle and 0.3％TWE correspondingly.A skin physiological monitor was used to measure transepidermal wa-ter loss(TEWL),stratum corneum hydration and skin surface pH value before treatment,as well as on day 2,4,6,8,10,12 and 14 after continuous topical administration.Fifteen C57BL/6J mice were randomly divided into a normal control group,vehicle group and 0.3％TWE group.Quantitative real-time polymerase chain reaction was applied to detect the mRNA expression level of filaggrin in the mouse epidermis of each group.Results In the irritant contact dermatitis mod-el,topical TWE significantly improved stratum corneum hydration levels and reduced transepider-mal water loss rates starting from day 4(P＜0.001),and reversed the TPA-induced elevation in skin surface pH from day 8(P＜0.001).In the allergic contact dermatitis model,topical TWE markedly increased stratum corneum hydration levels(P＜0.001)and decreased transepidermal water loss rates(P＜0.05)from day 4,while no significant alteration was observed in skin sur-face pH throughout the experiment.Compared with the normal control group,topical TWE signifi-cantly upregulated the mRNA expression of filaggrin in mouse epidermis(P＜0.001).Conclu-sions Topical TWE helps repair the epidermal permeability barrier in dermatitis mice,and re-store skin hydration levels and acidity.Its underlying mechanism may be related to the alleviation of inflammation-mediated inhibition of filaggrin gene transcription by TWE.