拉曼光谱激光诱导牙本质荧光背景的物质来源及过氧化物漂白剂对其影响机制的研究OA

Objective:To analyze changes in laser-induced fluorescence background following peroxide bleaching a-gent treatment using Raman spectroscopy and related techniques,identify the source of fluorescence variations.Methods:Fif-ty-four dentin sections were randomly assigned to three groups:10%neutral urea peroxide,40%neutral hydrogen peroxide,and deionized water control(n=18 each),with 24-hour reagent exposure.Raman and infrared spectroscopy were performed before and after treatment.Scanning electron microscopy examined dentin surface morphology,and non-collagen proteins were extracted for gel electrophoresis and mass spectrometry analysis.Results:Raman spectroscopy showed stable phosphate peaks while laser-induced fluorescence background intensity significantly decreased in both bleaching groups.Infrared spectroscopy demonstrated marked decline in collagen amide peaks.Scanning electron microscopy confirmed intact collagen fiber morphol-ogy.SDS-PAGE revealed marked reduction in non-collagen protein content,and mass spectrometry identified significant dif-ferential expression of non-collagen proteins associated with extracellular matrix remodeling and protein degradation.Conclu-sion:Dentin non-collagen proteins,rather than collagen,are the primary source of laser-induced fluorescence background;their oxidative degradation by peroxide bleaching agents directly reduces this background,providing molecular-level insight into tooth bleaching mechanisms.