木犀草素促进人成骨样细胞U2OS成骨分化的实验研究OA

Objective:To investigate the effects of luteolin on the proliferation,migration,and osteogenic differentia-tion capacity of human osteoblast-like U2OS cells,and to preliminarily explore its possible pro-osteogenic mechanism.Meth-ods:The proliferative activity of U2OS cells treated with different concentrations of luteolin for 48 h was assessed using the CCK-8 assay.The effect of 1 μmol/L luteolin on cell migration was evaluated using a Transwell chamber assay.Mineralized nodule formation was observed by Alizarin Red staining and semi-quantitative analysis.Alkaline phosphatase(ALP)activity was measured using an ALP activity assay kit after treatment with luteolin for 5 and 7 days.Furthermore,qRT-PCR was per-formed to detect the mRNA expression levels of the osteogenesis-related genes RUNX2 and COL-I.Results:The CCK-8 assay showed that low concentrations of luteolin had no obvious effect on the proliferation of U2OS cells.The proliferative activity of cells in the 0.5 μmol/L and 1 μmol/L treatment groups exhibited an increasing trend,the difference of 1 μmol/L was statis-tically significant.The Transwell assay demonstrated that treatment with 1 μmol/L luteolin significantly increased the number of migrated U2OS cells(P<0.01),indicating markedly enhanced migratory capacity.Alizarin Red staining showed that calci-um nodule formation was markedly increased in the luteolin-treated group,and the level of mineralization was significantly higher than that in the control group(P<0.01).ALP activity assays showed that luteolin treatment significantly increased cel-lular ALP activity after 5 and 7 days(P<0.05).qRT-PCR results revealed that the mRNA expression levels of RUNX2 and COL-I were significantly upregulated in the luteolin-treated group(P<0.05).Conclusion:Luteolin may promote osteoblast-like differentiation of U2OS cells by upregulating osteogenesis-related molecules such as RUNX2 and COL-I,indicating its potential application value in bone regeneration and bone tissue engineering.