荔枝炭疽病生防木霉菌的筛选及室内防效研究OA

Anthracnose,caused by the fungal pathogen Colletotrichum gloeosporioides,is a major disease affecting litchi production.Current control strategies predominantly rely on chemical fungicides.However,with increasing environmental concerns and the emergence of fungicide resistance,developing efficient and environmentally friendly biocontrol technologies is of great significance.In this study,strain YN012,which exhibited significant antagonistic activity against C.gloeosporioides,was screened from a collection of 19 Trichoderma isolates.In dual-culture assays,its inhibition rates via non-volatile metabolites,volatile metabolites and fermented filtrate reached 72.96%,78.14%,and 98.05%,respectively.Based on morphological characteristics and molecular identification,strain YN012 was identified as Trichoderma asperellum.The fermentation filtrate of this strain demonstrated a more pronounced inhibitory effect.At the concentration of 20%,it completely inhibited the formation of conidia.When the concentration was increased to 50%,it achieved inhibition rates exceeding 96%against both mycelial growth and conidial germination.In in vitro assays,treating litchi leaves and fruits with the 40%fermentation filtrate reduced the incidence of anthracnose to 29.00%and 25.33%,respectively.These findings provide a promising candidate strain for the development and application of strain YN012 as a biocontrol agent against litchi anthracnose.