散囊菌目新科、新属和新种——基于全基因组测序技术的9基因系统发育分析OA

Species of Eurotiales are of great industrial,agricultural,ecological and medicinal importance.A strain of this order XCW_SN069 was isolated from Guangdong Province of China.In order to determine its phylogenetic position,the whole genome of the strain was sequenced using high-throughput sequencing platform.Two genomes of Elaphomycetaceae and two others of Onygenales were retrieved from GenBank for phylogenetic comparison.Nine gene partitions were extracted from the genomes or gathered from the public databases,which include internal transcribed spacer(ITS),large subunit(28S)and small subunit(18S)of the nuclear ribosomal DNA,RNA polymerase Ⅱ second largest subunit(RPB2),RNA polymerase Ⅱ largest subunit(RPB1),theta subunit of the TCP-1 chaperonin complex(Cct8),a putative ribosome biogenesis protein(Tsr1),β-tubulin(BenA)and calmodulin(CaM).Separate and concatenated datasets were compiled containing all the 27 accepted genera of the five families for reconstruction of the phylogenies of Eurotiales.The strain XCW_SN069 represents a new species(Austromyces sinicus)of a new genus(Austromyces)belonging to a new family(Austromycetaceae)which is sister to the ectomycorrhizal family Elaphomycetaceae in the nine-gene phylogeny.The current concept of Thermoascaceae was not supported by the analysis and needs to be emended,thus another new family,Paecilomycetaceae was proposed to accommodate the genus Paecilomyces.The classification of Eurotiales was therefore updated to include seven families:Aspergillaceae,Austromycetaceae,Elaphomycetaceae,Paecilomycetaceae,Penicillaginaceae,Thermoascaceae and Trichocomaceae.Detailed description and illustration of the new species were given.Taxonomic comments for the new taxa at higher ranks were also provided.