柑橘间座壳菌全基因组非经典分泌蛋白预测及致病相关性分析OA

Citrus melanose,caused by Diaporthe citri,is one of the important diseases affecting citrus cultivation worldwide,which significantly reduces the appearance quality and economic value of citrus.Secretory proteins play crucial roles in the pathogen infection process.However,current research mainly focuses on classical secretory proteins,with limit-ed attention paid to non-classical secretory proteins.In this study,based on the whole-genome sequence of D.citri,non-classical secreted proteins were predicted by using TMHMM 2.0,WoLF PSORT,SignalP 5.0 and Secre-tomeP-1.0,and their functional annotations were analyzed by using dbCAN,eggNOG-mapper and PHI-BLAST.Mo-reover,the transcription levels of 11 candidate genes dur-ing D.citri infection were analyzed by using quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR).The results showed that 302 candidate non-classical secretory proteins were predicted from the 15 761 encoded proteins,of which 11 were potential virulence-enhancing proteins.Additionally,two candidate genes(INS49_010083 and INS49_015019)were significantly upregulated during the infection of fruits of the susceptible germplasm Cocktail grapefruit by Diaporthe citri,suggesting that they may be involved in the pathogenic process of D.citri.These results provide a theoretical basis for elucidating the pathogenic mecha-nisms of D.citri and conducting precision breeding of citrus varieties resistant to citrus melanose.