向日葵COBRA家族基因的全基因组鉴定与分析OA

COBRA proteins are a class of glycosyl-phosphatidylinositol(GPI)-anchored proteins that play important roles in plant cell wall biosynthesis,cell division,cell expansion,and defense responses.In this study,16 COBRA genes un-evenly distributed across 11 chromosomes were identified in the sunflower whole-genome.Based on bioinformatics analysis,this family was classified into two subfamilies,with significant differences in structural features,including gene structure,variations in the key conserved domain(cysteine-cysteine-valine-serine,CCVS),divergence in hydrophobicity/hydrophilicity patterns,and divergence in GPI-anchoring modification sites.Analysis of promoter cis-acting elements revealed that all mem-bers contained at least two types of cis-acting elements involved in stress response,hormone signaling,or developmental regulation.The protein-protein interaction prediction results showed that both HaCOBRA10 and HaCOBRA14 interacted with two cellulose synthases(CE-SA4,CESA8)and two glycosyltransferases(IRX3,IRX9).Tissue-specific expression and stress response analyses sug-gested that genes in this family may exhibit functional diver-sity in growth,development,and stress adaptation.This study provides new targets for research on cell wall formation mechanism and stress resistance breeding in sunflower,and lays a theoretical foundation for further investigation into the biological functions of COBRA proteins in plants.