小麦TaXI-III互作蛋白的筛选及其与TaHRLI-2B的互作分析OA

In order to further explore the molecular mechanism of the wheat xylanase inhibitor gene TaXI-III in re-sistance to Fusarium graminearum,this study cloned the TaXI-III gene from the moderately resistant variety Annong 1589.Bioinformatics and subcellular localization analysis revealed that the TaXI-III protein was localized to the cell membrane/wall.The bait vector was constructed by homologous recombination method.The self-activation and functional assays con-firmed that pBT3N-TaXI-III had no self-activation activity in NMY51 yeast cells,and could produce functional proteins.A total of 19 candidate proteins that may interact with TaXI-III were finally obtained by split-ubiquitin yeast two-hybrid system from wheat cDNA library.These proteins were associated with diverse biological functions,including signal transduction,transmembrane transport,programmed cell death,and stress response.Results from yeast retransformation,luciferase complementation assay and bimolecular fluores-cence complementation assay confirmed that TaHRLI-2B,an HR-like lesion-inducing protein involved in the hyper-sensitive response,interacted with TaXI-III in both yeast and plant cells.The expression of TaHRLI-2B was induced by Fusarium graminearum infection through quantitative real-time reverse transcription polymerase chain reaction(RT-qPCR)analysis.And the overexpression of TaHRLI-2B in Arabidopsis thaliana enhanced the resistance of plants to pathogens.The results of this study provide a theoretical foundation for further exploring the functional mechanism of TaXI-III and discovering disease-related genes in wheat.