普通小麦TaSeBP基因表达与自然等位变异分析OA

This study applied bioinformatics and molecular biology methods to analyze the expression and natural al-lelic variation of the wheat SeBP gene,providing theoretical basis and methods for screening and developing selenium-rich wheat germplasm resources.Through the analysis of expression levels of SeBP gene in different wheat varieties and studies under stress treatments,the results showed that TaSeBP-3D had the highest expression level.Furthermore,TaSeBP respon-ded to selenium supplementation and cold stress,with Huaimai 18 showing an upregulation in relative expression,while Jinghe No.1 showed a decrease in relative expression.Analysis of cis-acting elements in the candidate promoter region re-vealed the presence of abscisic acid-responsive,gibberellin-responsive elements,suggesting a relationship with plant growth and development and response to stress.DNA variation analysis in natural wheat populations indi-cated that TaSeBP had multiple missense mutation sites and single nucleotide insertion-deletion variations.Phylo-genetic and evolutionary analysis of wheat SeBP revealed differentiation between monocotyledonous and dicotyledon-ous plants.The results of this study can provide a reference for in-depth research on TaSeBP gene function and its applica-tion in molecular breeding.