基于非靶向代谢组学解析不同水稻品种的品质差异OA

To compare the differences in the composition of quality-related metabolites in rice among varieties stored at the same temperature,untargeted metabolomics approach based on ultraperformance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to analyze differential metabolites and elucidate metabolic pathways in the in-dica rice varieties,Taoxiangyou 188(TXY188)and Wuyou 308(WY308),before storage and after 90 d of storage under constant-temperature conditions.The results showed that a total of 3 055 metabolites were detected by UPLC-MS/MS.At the initial stage of storage,239 metabolites were accumulated at higher levels in TXY188 than in WY308,and metabolites such assyringetin,vanillin,cinnamic acid,and coniferaldehyde were significantly enriched in TXY188.After 90 d of storage,the contents of metabolites such as vanillin,cinnamic acid and coniferaldehyde remained higher in TXY188 than in WY308.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis revealed that flavonoid biosynthesis and phenylpropanoid metabolism were the major metabolic pathways involved.This study provides data support for regulation of grain quality during rice storage from the per-spective of dynamic changes in flavor-related metabolites.