植物乳杆菌胞外囊泡对DSS诱导结肠炎的缓解作用OA

In order to explore the ameliorative effects of Lactobacillus plantarum extracellular vesicles(LpEVs)on colitis,extracellular vesicles extracted from L.plantarum of porcine source were administered to C57BL/6 mice at different concentrations,and then DSS was used to induce colitis model.The weight change rate of mice,the disease activity index,the length of the colon,the spleen index,and the pathological changes of the colon tissue were monitored,and the activity of myeloperoxidase in serum and the levels of inflammatory cytokines(IL-6,IL-1β and TNF-α)were detected by ELISA.The results showed that the body weight of DSS-induced colitis mice decreased,the DAI index increased,the colon shortened,the spleen index increased,the colon tissue was seriously damaged,the serum myeloperoxidase activity increased,and the expression levels of pro-inflammatory cytokines IL-6,IL-1β and TNF-α increased.After intervention with LpEVs,all the above indicators were improved,indicating that L.plantarum extracellular vesicles have a good alleviating effect on colitis.The research results provide a theoretical basis for exploring the mechanism by which probiotics alleviate colitis.