miR-221与miR-802在前列腺癌术后复发中的交互作用及其预测效能OA

Objective To explore the interaction between microRNA-221(miR-221)and miR-802 in postoperative recurrence of prostate cancer,as well as the predictive efficacy of the two indicators alone and in combination for postoperative recurrence.Methods A total of 105 patients with prostate cancer who underwent radical prostatectomy at Xinxiang Central Hospital from January 2018 to May 2023 were retrospectively enrolled.All patients were followed up until October 31,2025,with a median follow-up time of 26.5 months.The expression levels of miR-221 and miR-802 in cancer tissues were compared between patients with and without recurrence.According to the levels of miR-221 and miR-802,the patients were divided into four groups:Q1-Q4.Clinical data and postoperative recurrence rates were compared among the four groups.Cox regression analysis was used to analyze the relationship between the levels of miR-221 and miR-802 in cancer tissues and postoperative recurrence,as well as their interaction on postoperative recurrence.The efficacy of miR-221 and miR-802 levels in predicting postoperative recurrence of prostate cancer was further evaluated.Results Compared with patients without recurrence,patients with recurrence had significantly higher miR-221 levels and lower miR-802 levels in cancer tissues(P<0.05).There were statistically significant differences in tumor stage,Gleason score,prostate-specific antigen(PSA)level,lymph node metastasis,extent of lymph node dissection,postoperative radiotherapy,and postoperative hormone therapy among the four groups(P<0.05).The postoperative recurrence rates in Q1 to Q4 groups were 9.7%(3/31),36.0%(9/25),37.5%(9/24)and 68.2%(15/22),respectively,showing a gradually increasing trend(P<0.05).Cox regression analysis showed that miR-221 and miR-802 were independent influencing factors for postoperative recurrence of prostate cancer(P<0.05),and no multicollinearity was observed(variance inflation factor<10).The goodness-of-fit test indicated that the model exhibited good fitness.Both miR-221 and miR-802 were independent influencing factors for postoperative recurrence of prostate cancer,and high miR-221 level combined with low miR-802 level exerted a positive interaction on postoperative recurrence of prostate cancer(P<0.05).The area under the ROC curve(AUC)of the combination of miR-221 and miR-802 in cancer tissues for predicting postoperative recurrence was significantly higher than that of either indicator alone(P<0.05).Internal validation results using the Bootstrap resampling method(1000 replications)showed that the concordance index(C-index)of the combined prediction of miR-221 and miR-802 was close to the original AUC.The calibration curve demonstrated that the recurrence probability predicted by the combination of the two indicators was highly consistent with the actually observed recurrence probability.Conclusions The expression levels of miR-221 and miR-802 in prostate cancer tissues exhibit an interaction on postoperative recurrence,and the combination of the two indicators can provide a reference for clinical prediction of postoperative recurrence in prostate cancer.