两个海区福建牡蛎糖原相对高低群体代谢组差异分析OA

Oysters are the most productive mariculture shellfish in China,with the national aquaculture output reaching 7,252,500 tons in 2024.Among them,Crassostrea angulata is the main species cultured in Fujian Prov-ince.Glycogen content is a crucial,quality-associated trait that considerably affects oyster flavor.However,re-search on the mechanisms regulating glycogen content in C.angulata is limited,hindering progress in genetic im-provement.In this study,C.angulata from a single population were cultured in two sea areas,Meizhou(Putian)and Shijing(Quanzhou).Metabolomic analysis was performed on individuals from each area with notable variations in glycogen content.After 10 months of culture,these differences were highly notable,with the maximum variations being in the multiples of 17.42 and 8.79,respectively.Compared with the high-glycogen group,189 and 361 dif-ferentially accumulated metabolites were identified in the low-glycogen groups from the two sea areas,respectively.The commonly enriched metabolic pathways included arachidonic acid metabolism,neuroactive ligand-receptor interaction,and the FOXO signaling pathway.In addition,the D-amino acid metabolism pathway was enriched in the specimens from Meizhou,while the glycerophospholipid metabolism pathway was enriched in the specimens from Shijing.C.angulata may affect glycogen content through multiple pathways:upregulating the expression of LXA4 to inhibit inflammatory response,reducing energy consumption,and promoting glycogen storage,enhancing gluconeogenesis and other processes through the D-amino acid and starch-sucrose metabolism pathways to produce more glucose for glycogen biosynthesis.This study provides basic data for the subsequent genetic improvement of glycogen-related traits in C.angulata.