代谢组学揭示60Co-γ与电子束辐照对红参的影响OA

In order to investigate the effects of 60Co-γ irradiation and electron beam irradiation on the chemical constituents of red ginseng and further optimize its irradiation processing,red ginseng powder was treated at the absorbed dose ranging from 0 to 100 kGy in this study.A non-targeted metabolomics approach based on the ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS)combined with multivariate statistical methods such as the principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)was used to systemically analyze the chemical composition changes and screen for differential markers in red ginseng samples.A total of 59 chemical compounds were identified,including 48 ginsenosides.The results demonstrated that the electron beam irradiation caused less disturbance to the red ginseng metabolome,whereas 60Co-γ irradiation induced significant changes in 37 metabolites,of which 34 were ginsenosides,showing a clear dose-dependent pattern.Both irradiations exerted limited effects on the contents of major ginsenosides(Rg1,Re,and Rb1).Overall,this study revealed that the electron beam irradiation has a milder impact on red ginseng constituents compared with 60Co-γ irradiation,and it provides scientific evidence for optimizing the irradiation processing and quality control of red ginseng products.