基于代谢组学和转录组学的韦氏曲霉侵染葡萄研究OA

To investigate the metabolic characteristics and potential biomarkers of Aspergillus westerdijkiae after infecting grape berries.Summer Black grapes were inoculated with strain fc-1 and incubated at 28℃for 7 d.Non-targeted metabolomic analysis were performed using high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(HPLC-QTOF-MS/MS),and Pearson correlation analysis was conducted between differential metabolites and differentially expressed genes identified from transcriptomic data.A.westerdijkiae successfully colonized the grape surface and induced visible lesions,showed great differences from those grown on potato dextrose agar.A total of 146 differential metabolites were identified,mainly including lipids,organic acids and sugar alcohols.These metabolites were enriched in the lysine degradation,galactose metabolism and fatty acid biosynthesis pathways.Inositol and dulcitol were significantly up-regulated and positively associated with TU_AoFC_00318(encoding the urea amidolyase α-subunit),TU_AoFC_02007(encoding methionine transporter),TU_AoFC_09517(encoding endo-1,6-α-mannanase),and TU_AoFC_10219(encoding polyketide synthase),while showing significant negative correlations with TU_AoFC_00835/09062(encoding copper transporters),TU_AoFC_01162(encoding dehydrogenase),and TU_AoFC_08458(encoding transporter).The results of this study provide a scientific basis for elucidating the pathogenic mechanism of A.westerdijkiae and for establishing a metabolism-based strategy for the prevention,control,and early warning of grape diseases.