发根农杆菌介导的CsMYBS1转化黄瓜及耐盐性分析OA

To investigate the function of the transcription factor CsMYBS1 in the salt stress response of cucumber,cucumber seedlings were used as the experimental material for transformation.First,the plant overexpression vector pCAMBIA2300s-GFP:CsMYBS1 was constructed.Subsequently,the empty vector plasmid and the pCAMBIA2300s-GFP:CsMYBS1 plasmid were individually transformed into Agrobacterium rhizogenes strain K599.Using the hypocotyl injection method,we obtained CsMYBS1-overexpressing(CsMYBS1-OE)hairy root chimeric plants and empty vector(EV)control hairy root chimeric plants.Under 50 mmol·L-1 NaCl stress treatment,the phenotypic differences between the two types of chimeric plants were observed,and the dynamic changes in malondialdehyde content,soluble sugar,soluble protein,proline content,and antioxidant enzyme activity in plant leaves were measured.The results showed that under salt stress,compared with EV control chimeric plants,the leaf chlorosis and scorching symptoms of CsMYBS1-OE chimeric plants were significantly alleviated,and the root system was more developed.The increase in soluble sugar and proline content in the leaves of CsMYBS1-OE chimeric plants was more gradual.Malondialdehyde content was significantly reduced in CsMYBS1-OE chimeric plants and was markedly lower than that in the EV chimeric plants.The catalase activity increased significantly in CsMYBS1-OE chimeric plants,whereas that in EV plants continuously decreased.The peroxidase activity in CsMYBS1-OE chimeric plants continued to increase,but at a relatively gradual rate.In summary,this study utilized Agrobacterium-mediated hairy root technology to demonstrate that CsMYBS1 can enhance cucumber salt tolerance by coordinating osmotic regulation and oxidative homeostasis,which lay a foundation for further investigation into the CsMYBS1 regulatory network and genetic improvement of cucumber.