陆地棉单糖转运蛋白基因的鉴定及功能验证OA

Monosaccharide transporters(MSTs)are key regulators of sugar transport and signaling,yet they have not been systematically characterized.To clarify their roles in Verticillium wilt resistance,we identified the MST gene family from the whole genome of Gossypium hirsutum using bioinformatic methods,and performed analyses of chromosome localization,phylogenetic relationships,conserved motifs,gene structures,collinearity,and expression patterns.The functions of two family members,GhMST36 and GhMST67,were further validated using virus-induced gene silencing(VIGS).A total of 126 GhMST genes were identified,unevenly distributed across 26 chromosomes and classified into seven subfamilies.These genes encode proteins 3-17 transmembrane domains and possess 1-18 exons,with members of the same subfamily sharing similar conserved motifs and exon compositions.Collinearity analysis revealed 132 collinear relationships among GhMST members,which were organized into seven gene clusters.Expression profiling showed that 12 GhMSTs were up-regulated following infection by Verticillium dahliae.Plants silenced for GhMST36 or GhMST67 exhibited milder disease symptoms,along with lower disease indices and reduced fungal biomass after infection with V.dahliae,indicating that they function as negative regulators of cotton resistance to Verticillium wilt.This study provides important theoretical support for the molecular breeding of disease-resistant cotton.