甘薯PIN基因家族鉴定和非生物胁迫下的表达分析OA

The auxin efflux transporter PIN is a key factor mediating auxin polar transport and maintaining auxin concentration gradients,playing a crucial role in plant growth,development,and abiotic stress responses.To investigate the biological functions of the PIN gene family in sweetpotato(Ipomoea batatas),this study employed an integrated bioinformatics and transcriptomics approach to identify its family members and analyze their response patterns to abiotic stresses in sweetpotato.The results showed that a total of 17 PIN family genes(IbPIN1-IbPIN17)were identified in the sweetpotato genome,which are unevenly distributed across 9 chromosomes.All their encoded proteins contain 1-4 conserved Mem_trans domains and are predominantly localized to the plasma membrane.Phylogenetic analysis classified this family into 6 subclades and revealed 9,22,and 21 collinear gene pairs between sweetpotato with Arabidopsis thaliana,Ipomoea triloba,and Ipomoea trifida,respectively.Promoter cis-element analysis indicated that these genes contain a total of 38 cis-acting elements associated with stress response,growth and development,hormone response,and light response.Transcriptome profiling demonstrated that IbPIN genes are actively involved in the response to abiotic stress.Among them,the expression of IbPIN2,IbPIN3,IbPIN4,IbPIN5,IbPIN7,and IbPIN16 was significantly upregulated under heat stress,whereas their expressions were markedly suppressed under drought or salt stress.These findings suggest that these six genes may play a key role in regulating the response of sweetpotato to adverse environmental conditions.This study provides a systematic reference for functional characterization of sweetpotato PIN genes and offers valuable candidate gene resources for breeding stress-resistant sweetpotato varieties.