花生衣通过调节MAPK/PI3K/AKT信号通路缓解庆大霉素诱导的急性肾损伤OA

Objective:To explore the effect and mechanism of peanut skin extract in improving acute kidney injury.Methods:Based on UPLC-Q TOF-MS technology,peanut skin extract and its blood components were analyzed.Target prediction was per-formed on the blood components of peanut skin extract and acute kidney injury disease through a database.The intersection targets were selected to construct a"drug-active ingredient-target-disease pathway"network.GO and KEGG functional enrichment analy-sis were used to analyze the network pathways and screen for core active ingredients with high correlation.An acute kidney injury model was established by intraperitoneal injection of gentamicin.Pathological tissue sections were used to detect the degree of re-nal inflammatory cell infiltration.Microplate method was used to detect renal function indicators,inflammatory factor content,and renal tissue oxidation indicators.Western blot was used to detect the expression levels of key proteins in the MAPK/PI3K/AKT signaling pathway.Results:Based on UPLC-Q TOF-MS technology,45 chemical components and 14 blood entering components were identified from peanut skin extracts.A network was constructed to obtain 131 intersecting targets.GO and KEGG enrich-ment analysis mainly involved the MAPK signaling pathway,PI3K signaling pathway,and AKT signaling pathway.The core components are Arachidonic Acid Linoleic Acid,Esculetin,7-Hexadecenoic acid,and Daidzein.Peanut skin extract can signifi-cantly reduce the levels of BUN and CRE in rat serum induced by gentamicin,as well as decrease the levels of UP,LZM en-zymes,and NAG in urine.Reduce the infiltration of inflammatory factors such as TNF-α,IL-1β,and IL-6,lower the levels of NO,NOS,and MDA,and increase the levels of GSH,SOD,and UA.Western blot validation showed that peanut skin extract effectively inhibited apoptosis of renal tubular epithelial cells by downregulating the phosphorylation levels of key proteins in the MAPK/PI3K/Akt pathway.Conclusion:Peanut skin extract alleviates acute kidney injury induced by gentamicin through the MAPK/PI3K/AKT signaling pathway,providing theoretical support for the active ingredients and pharmacological mechanisms of peanut skin in alleviating acute kidney injury.