缺氧相关基因FHDC1对肝癌细胞增殖、侵袭转移及糖酵解能力的影响OA

Objective:To investigate the effects of hypoxia-related gene FH2 domain containing 1(FHDC1)on progression,migration,invasion and glycolysis of hepatocellular carcinoma(HCC)cells.Methods:Western blot was used to detect the protein expression of HIF-1α and FHDC1 in HepG2 cells after hypoxia-inducible factor 1-α(HIF-1α)knocked out.Hepatoma cell lines HepG2 and Hep3B with stable overexpression and knockout of FHDC1 were constructed by lentiviral transfection,respectively.CCK-8,plate colony formation assay,Transwell migration and invasion assay were used to detect the effects of FHDC1 on the proliferation,migration,and invasion of HepG2 and Hep3B cells under normoxia and hypoxia.Western blot was used to investi-gate the effects of FHDC1 under normoxic and hypoxic conditions on the expression of key glycolytic proteins in HepG2 and Hep3B cells:glucose transporter 1(GLUT1),pyruvate kinase M2(PKM2),and lactate dehydrogenase(LDHA).Results:The expression of FHDC1 was downregulated in HepG2 cells with HIF-1α-knockout under normoxia and hypoxia.Whether nor-moxia or hypoxia,compared to the corresponding control groups,overexpression of FHDC1 could enhance the proliferation,mi-gration,and invasion of HepG2 and Hep3B cells.Conversely,the proliferation,migration,and invasion of HepG2 and Hep3B cells were decreased after knockout of FHDC1.Under normoxia and hypoxia,the expressions of GLUT1,PKM2,and LDHA was increased in FHDC1-overexpressed HepG2 and Hep3B cells;While after knockout of FHDC1,the expressions of GLUT1,PKM2,and LDHA was decreased in HepG2 and Hep3B cells(P<0.05).Conclusion:HIF-1α regulates FHDC1 expres-sion in HCC cells.FHDC1,in turn,enhances the proliferation,migration,and invasion of HCC cells,and promotes the expresion of glycolysis-related proteins.