玉竹转录组测序及生物信息学分析OA

Objective To analyze the transcriptome expression characteristics of Polygonatum odoratum.Methods Total RNA was extracted from Polygonatum odoratum,and a cDNA library was constructed for high-throughput sequencing.Unigene sequences were obtained through de novo assembly.Genetic information at the transcriptome level was deciphered through sequence annotation and comparative analysis.Results A total of 76 335 unigenes were identified,with an average length of 807 bp and an N50 value of 1 245 bp.Among them,36 550 unigenes were functionally annotated in at least one database,and Polygonatum odoratum exhibited the highest sequence similarity to Asparagus officinalis.In the KEGG database,23 136 unigenes were annotated,including enzyme genes involved in terpenoid,flavonoid,and polysaccharide biosynthesis pathways,numbering 78,76,and 329,respectively.In the GO database,28 909 unigenes were annotated,covering categories such as cellular anatomical entity,binding,and cellular process.Furthermore,MISA analysis of 18 055 unigenes longer than 1 000 bp identified 7 623 simple sequence repeat(SSR)loci,and corresponding primers were designed.Additionally,by aligning the unigenes against the plantTFDB database,367 transcription factors including MYB,AP2/ERF,WRKY,and bHLH were identified.Conclusion The transcriptome data generated in this study provide a crucial foundation for in-depth exploration of the biosynthetic pathways and functional genes of medicinal components in Polygonatum odoratum,and also support its resource conservation and development.