熵驱动介导传感器在miRNA检测中的研究进展OA

MicroRNAs(miRNAs)are important non-coding RNA molecules,whose expression levels play a significant role in various physiological and pathological processes.The entropy-driven reaction(EDR)offers a novel method for amplifying miRNA signal into more stable DNA signal through a miRNA-initiated cyclic process,all steps under non-enzymatic catalysis and isothermal conditions.This approach not only facilitates the direct detection of DNA but also enables subsequent analyses utilizing the DNA products generated in the process.In contrast to conventional miRNA analysis techniques,EDR is operated without specialized reagents or equipment,solely harnessing entropy changes within the system to achieve necessary sensitivity and selectivity.Additionally,the EDR process is straightforward and flexible.Based on these characteristics,a range of innovative miRNA sensing strategies and detection methods have been developed utilizing EDR assay.This review summarized three major research directions in miRNA detection,including direct EDR-based detection,EDR combined with isothermal amplification,and EDR combined with nanomaterials,in which their typical applications and advantages were discussed.Finally,this review highlighted the current challenges and future development trends related to EDR-mediated miRNA biosensors,with the aim of providing a theoretical foundation for the development of more sensitive and accurate sensors in the future.