黄瓜miR166家族特征及其在干旱和盐胁迫下的表达分析OA

[Objective]This study aimed to analyze the characteristics of cucumber miR166 family members and their expression patterns under drought and salt stress,providing a theoretical basis for functional studies of cucumber miR166 family members.[Method]Based on miRNA sequencing data,cucumber miR166 family members(miR166s)were systematically identified,and their characteristic features were analyzed.miRNA se-quencing data and RT-qPCR techniques were used to analyze the expression of cucumber miR166s under drought and salt stress.[Result]Fifteen miR166s were clustered into three evolutionary branches and distributed across five cucumber chromosomes,with their mature sequences being relatively conserved in phylogeny.Most miR166s responded to drought stress at early stages with relatively stable expression levels,while some miR166s showed high or extremely high expression under salt stress.For example,MIR166c-p5 exhibited extremely high expres-sion under salt stress,and miR166e_L+2R-1 and miR166a-3p_R+2 were highly expressed under salt stress,indi-cating that miR166 family members may play important roles in cucumber's response to drought and salt stress.[Conclusion]miR166 family members are involved in cucumber's response to drought and salt stress processes.