'巴梨'炭疽病病原菌的分子鉴定及其生防菌筛选OA

[Objective]Pear anthracnose,caused by Colletotrichum species,severely affects pear leaves and fruits,leading to significant economic losses.To effectively control this disease,this study aimed to identify the pathogenic spe-cies responsible for anthracnose in European pears and screen for effective biocontrol yeasts.[Method]Three Colleto-trichum isolates(Bali-1-Bali-3)were purified and identified through pathogen isolation and purification,multilocus phy-logenetic analysis,and pathogenicity tests.[Result](1)Bali-1 was identified as Colletotrichum siamense,while Bali-2 and Bali-3 were identified as Colletotrichum fructicola.(2)Two biocontrol yeast strains,Hanseniaspora uvarum(Huv1)and Metschnikowia pulcherrima(Mp1),were selected and both showed significant inhibitory effects against the three patho-genic strains.(3)Plate inhibition assay results indicated that both Huv1 and Mp1 exhibited good inhibitory effects against the three pathogens.Specifically,the inhibition rates of Huv1 were 38.9%,40.7%,and 44.2%,respectively,while those of Mp1 were 34.6%,8.7%,and 27.7%,respectively.The fruit efficacy test showed that Huv1 and Mp1 inhibited the growth of the pathogens Bali-1 and Bali-2 to varying degrees.[Conclusion]This study clarifies that the dominant pathogens caus-ing anthracnose in'Bartlett'pears are C.siamense and C.fructicola,and identifies two promising biocontrol yeast strains,Huv1 and Mp1,providing a theoretical basis and microbial resources for the biological control of pear anthracnose.