鲜食型甘薯品种'普薯32号'脱毒苗表型及品质性状分析OA

[Objective]This study aimed to obtain virus-free seedlings of the main sweet potato varieties in Fuyang City,Anhui Province,and analyze the differences in phenotype,yield,and quality between them and the virus-carrying seedlings.[Method]Viral detection was conducted to confirm the presence of viral infection,and the virus-free seedlings of'Pushu 32'were obtained by treating infected plants with meristem culture;the pheno-typic traits and yield characteristics of both the virus-free'Pushu 32'seedlings and the infected plants were ana-lyzed under both greenhouse and field conditions.[Result](1)The virus infection rates of four major sweet potato cultivars in Fuyang City,Anhui Province varied significantly.(2)The regenerated plants of'Pushu 32'were suc-cessfully obtained,and RT-PCR analysis confirmed that all four viruses carried by this cultivar had been eradicat-ed.(3)Greenhouse-grown virus-free'Pushu 32'seedlings exhibited superior morphological traits and root mass compared to infected plants.Additionally,the net photosynthetic rate of virus-free seedlings was higher than that of the infected plants.(4)Field-grown virus-free'Pushu 32'seedlings demonstrated superior phenotypic traits and yield structure indicators at harvest.Furthermore,the virus-free seedlings showed higher dry matter content,starch content,soluble sugar content,and reducing sugar content than the infected plants.[Conclusion]Virus-free seedlings of'Pushu 32'were successfully obtained,which showed superior phenotypic traits,yield,and quality indicators compared to virus-infected plants,achieving the goal of purification and rejuvenation.